Higher Education (HE) in Iran have been subject to a major expansion and massification in the recent years, in a way that number of students approximately tripled from 2006 to 2016. This would have possibly affected labor market or unemployment rate of the country. Considering both provincial and national level, this study investigates the relationship between HE expansion and unemployment rate in the recent era (2006-2018) empirically. In this regard, number of assignments, students, and the state budget allocated to HE institutions are taken as variables indicating HE expansion so that their relationship with unemployment rate can be explored. The empirical methodology of this study in national level is to consider trends and calculating correlations for different lags. In provincial level, Granger causality and dynamic panel data regression with systemic GMM estimators are utilized as methods of the analysis. The results show a positive significant correlation exists between the state budget of HE and unemployment rate. Moreover, in provincial level, number of students and assignments Granger cause unemployment in some lags. Dynamic panel data model with numerous specifications also approve a positive significant relationship between HE expansion in provinces and their unemployment rate, however, the effect is not the same considering different models, especially for number of students.